Table 1. Pangenomic HCV RNA detection.
Viral RNA from the six HCV genotypes at various input copy numbers were amplified via two-step RT-RPA. Genotyped viral RNA was extracted from HCV-positive human serum and spiked into reverse transcription and RPA reactions for detection. The lowest LODs were observed for genotypes 1 and 4. Genotypes 3 and 6 were the most challenging to detect with inconsistent amplification observed at 100 copies per reaction or less. All no template controls (NTCs) did not amplify in RPA (N = 28).
| HCV Genotype | cp/rxn | # positives |
|---|---|---|
| 1 | 500 | 4/4 |
| 100 | 4/4 | |
| 50 | 4/4 | |
| 25 | 4/4 | |
| 10 | 1/4 | |
| 2 | 500 | 4/4 |
| 100 | 4/4 | |
| 25 | 3/4 | |
| 10 | 0/4 | |
| 3 | 500 | 4/4 |
| 100 | 3/4 | |
| 25 | 0/4 | |
| 4 | 75 | 6/6 |
| 15 | 5/6 | |
| 5 | 500 | 4/4 |
| 100 | 4/4 | |
| 50 | 0/4 | |
| 6 | 500 | 4/4 |
| 250 | 4/4 | |
| 100 | 0/4 | |
| negative | 0 | 0/28 |