Figure 1.

Overview of experimental paradigm, image processing pipeline and neuroanatomical regions and identify of target structures used for primary cultures. A, The brain of postnatal day 0–2 mouse pups were dissected, and target structures isolated before cell dissociation and culture in 96-well plates for 10 DIV. B, Overview of the eight target structures and subsequent dissection strategy in four transgenic mouse lines (TH-GFP, DAT-Ai9, ChAT-Ai9, and SERT-Ai9). Additional images are from the Allen Developing Mouse Brain Atlas (Lein et al., 2007; Allen Brain Atlas, 2008). TH, tyrosine hydroxylase; MAP2, microtubule associated protein 2; DAPI: 4′,6-diamidino-2-phenylindole; LC, locus ceoruleus; DMV, dorsal motor nucleus of the vagus; XII, hypoglossal nucleus; SNc, substantia nigra pars compacta; VTA, ventral tegmental area; SERT, serotonin transporter; DAT, dopamine transporter; ChAT, choline acetyl-transferase.