Figure 2.

Vulnerable neurons are more vulnerable to hydrogen peroxide than resilient neurons. Neurons were treated with hydrogen peroxide at 10 DIV, and fixed at 11 DIV. A, Example photomicrographs of all positive-identified neuron types across the vulnerable, and resilient target structures. B, Normalized number of positive neurons across hydrogen peroxide concentrations. Box and whiskers plots, in the style of Tukey, where the median value is indicated, and the lower and upper hinges correspond to the first and third quartiles. * = one-way ANOVA, Tukey’s HSD test, p < 0.05; * = pairwise t test, vulnerable versus resilient, p < 0.05. Detailed statistical tests and multiple comparisons can be found in Extended Data Table 2-1. TH, tyrosine hydroxylase; MAP2, microtubule associated protein 2; LC, locus ceoruleus; DMV, dorsal motor nucleus of the vagus; XII, hypoglossal nucleus; SNc, substantia nigra pars compacta; VTA, ventral tegmental area; R, raphé; STR, striatum.