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. 2022 Oct 24;9(5):ENEURO.0139-22.2022. doi: 10.1523/ENEURO.0139-22.2022

Figure 4.

Figure 4.

No overt difference in ROS production is observed between neurons. A, The redox sensitive GFP, roGFP, was expressed in a cre-dependent manner in target neurons. Photomicrographs of roGFP in a TdTomato-positive SNc DA neuron, in the somatodendritic (STD) compartment and in the axonal compartment. B, Example traces of GFP fluorescence arbitrary units (AU) in responsive ROIs, in an SNc DA neuron. Two traces are shown: for a GFP-positive puncta showing low relative oxidation status, and for a GFP-positive puncta showing high relative oxidation status. C, Quantification of the relative oxidative state of mitochondria across neuron types. Box and whiskers plots, in the style of Tukey, where the median value is indicated, and the lower and upper hinges correspond to the first and third quartiles, Kruskal–Wallis multiple comparison, p-values adjusted with the Bonferroni method, *p < 0.05. Shared control estimation plot: mean difference for comparisons against the shared control, SNc, using Data Analysis with Bootstrap Estimation, with 5000 bootstrap resamples. All confidence intervals are bias-corrected and accelerated. D, Quantification of intermitochondrial distance in the axonal domain (measured from the center of each GFP positive puncta). Box and whiskers plots, in the style of Tukey, where the median value is indicated, and the lower and upper hinges correspond to the first and third quartiles, Kruskal–Wallis p > 0.05. Density plots show distribution of individual measurements. Detailed statistical tests and multiple comparisons can be found in Extended Data Tables 4-1 and 4-2. Extended Data Figure 4-1 also provides micrographs showing the expression of MTSroGFP in locus coeruleus neurons and comparative data on the relative oxidation in the somatodendritic and axon domain of the different neuron types, as well as the normalized number of DAPI-positive nuclei in the neuron survival experiments performed with hydrogen peroxide. LC, locus ceoruleus; DMV, dorsal motor nucleus of the vagus; XII, hypoglossal nucleus; SNc, substantia nigra pars compacta; VTA, ventral tegmental area; R, raphé; STR, striatum.