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. 2022 Oct 13;31(19-20):604–620. doi: 10.1089/scd.2021.0279

FIG. 5.

FIG. 5.

Triple negative breast cancer enhances ASC inflammatory cytokine expression. (A–C) ASCs were stimulated with MCF-7, MDA-MB-231, and TU-BcX-4IC CM for 72 h and harvested for qRT-PCR analysis. Findings show significant P values (*P < 0.05) in SDF-1 and IL-8 expression in MCF-7 stimulated ASCs and IL-8 and MCP-1 expression in MDA-MB-231 stimulated ASCs. N = 3 biological (MCF-7 CM, MDA-MB-231 CM, N = 2 TU-BcX-4IC) and N = 2 technical replicates, error bars = SEM (D, E) ASCs were stimulated with culture medium, MCF-7 CM, or TNBC CM for 30 min and harvested for western blot analysis of selected inflammatory and cytokine pathways. FGF-1, fibroblast growth factor 1; JNK, Janus kinase; VEGF, vascular endothelial growth factor.