FIGURE 2.

Effect of the gene tlx1 and tlx2 deletion on the anti-Xoo activity of TlSMF2. (A) Growth of the WT TlSMF2 and its mutants on the test plates containing potato dextrose agar medium after cultured for 4 days. The picture shows a representative of three repeats. (B) The representative anti-Xoo activities of the WT TlSMF2 and its mutants on the test plates containing nutrient agar medium after co-cultured for 6 days. (C) The OD₆₀₀ of the co-cultures with the WT TlSMF2 and its mutants after 24 and 48 h. Control: Xoo cultured without WT TlSMF2 or its mutants. WT, the co-culture of Xoo with WT TlSMF2. Δtlx1, Δtlx2 and Δtlx1&tlx2, the co-cultures of Xoo with the mutant strains Δtlx1, Δtlx2 and Δtlx1& tlx2, respectively. (D) The representative Xoo-inhibition zones of the SMs extracted from WT TlSMF2 and its mutants on the test plate containing nutrient agar medium (left) and the diameters of the zones (right). a, b, c and d represent the SMs produced by strains WT TlSMF2 (a), Δtlx1(b), Δtlx2 (c) and Δtlx1&tlx2 (d), respectively. The diameters were data from three repeats (mean ± S.D.). Asterisk indicates significant difference compared with the control of MeOH (** means P < 0.01). (E) The growth of Xoo in nutrient broth medium containing the SMs (0.8%, v/v) extracted from the WT TlSMF2 and its mutants. MeOH, the nutrient broth medium containing methanol (0.8%, v/v). The graphs show data from triplicate experiments.