Fig. 5.

RGS5 does not regulate human brain pericyte proliferation under hypoxia. (A-C) EdU incorporation assay was performed to measure pericyte proliferation activity in siRGS5 and siCTRL pericytes. All experimental groups were exposed to hypoxia for 8 h and treated with EdU during the last hour of incubation. The percentage of EdU (APC-A)- and DAPI (BV421)-positive cells was used to measure pericytes in S-phase or G0/G1 and G2/M and sub-G1 with flow cytometry. Non-treated (A) or pericytes treated with S1P (1 μM) (B) or PDGFBB (5 ng/ml) (C) after 1 h in hypoxia were analysed. The data are presented as mean±s.d., n=3.