Table 2.

The results of fluorescence quantitative experimental analysis.

Gene	Ct value			Ct average	ΔCt	ΔΔCt relative copy number 2−△△Ct
	1	2	3
GlnR (0 mg/ml)	28.32	28.04	28.06	28,0.14	0	1
GlnR (1 mg/ml)	28.56	28.7	28.47	28.57	0.07	1.05
GlnR (3 mg/ml)	28.58	28.62	28.62	28.67	0.95	0.51
Nir (0 mg/ml)	32.51	31.63	31.95	32.03	0	1
Nir (1 mg/ml)	31.81	31.7	31.48	31.66	0.87	1.83
Nir (3 mg/ml)	32.72	32.47	32.39	32.49	0.88	0.54