Appendix 1—table 2. Verification of transgene homozygosity.
| Mosquito line | Larva numbers | Red | Yellow | Blue |
|---|---|---|---|---|
| Mg1 | 412 | 412 | ||
| Mg2 | 276 | 276 | ||
| Sg1 | 178 | 178 | ||
| Sg2 | 329 | 329 | ||
| E1 | 206 | 206 | ||
| E2 | 378 | 378 | ||
| Mg/E1 | 262 | 262 | 262 | |
| Mg/E2 | 198 | 198 | 198 | |
| Sg/E1 | 307 | 307 | 307 | |
| Sg/E2 | 345 | 345 | 345 | |
| Mg/Sg/E1 | 228 | 228 | 228 | 228 |
| Mg/Sg/E2 | 361 | 361 | 361 | 361 |
A total of 20 transgenic female mosquitoes from each line were mated with wild-type males and the progeny larvae assayed for expression of the dominant eye fluorescence marker. No non-fluorescent larvae were found, indicating that the females were homozygous for the transgenes.