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. 2022 Oct 12;11:e74270. doi: 10.7554/eLife.74270

Figure 12. Adult klc4uw314 mutant anxiety-like behavior does not fully habituate after repeat exposure to a novel environment.

(A) Percentage of fish per experiment that froze during the first 3 min of a 10-min initial exposure to a novel tank. (B) Plot of the percentage of fish that froze during the course of six 10-min exposures to the same novel tank. (C) Plot of the average time spent frozen during the course of six 10-min exposures to the same novel tank. (D) Plot of the average total and mobile-only swim velocities of fish during each of six 10-min exposures to the same novel tank. Mobile-only velocity excludes periods of time the fish spent at a velocity of 0 cm/s (frozen). (E) Plot of the percentage of time that fish spent in the upper zone of the tank during the course of six 10-min exposures to the same novel tank. (E) Plot of the average time it took for a fish to first enter the upper zone of the tank during the course of six 10-min exposures to the same novel tank. For all data, N=4 groups per genotype, 23 total fish per genotype. Error bars = SEM. For figures D-F, videos that could not be automatically tracked were omitted (for WT, 6/138, for klc4uw314, 4/138).

Figure 12—source data 1. The percentage of fish per group that froze during the first 3 min of exposure 1 of the habituation series.
Figure 12—source data 2. The percentage of fish per group that froze for each exposure in the habituation series.
Figure 12—source data 3. The total time spent frozen by each individual fish throughout the experimental time period for each exposure in the habituation series.
Figure 12—source data 4. The mobile and total swim velocities for each individual fish throughout the experimental time period for each exposure in the habituation series.
Figure 12—source data 5. The percentage of fish per group that entered the upper zone of the tank at least once during each exposure in the habituation series.
Figure 12—source data 6. The time in seconds it took each individual fish to initially cross over into the upper zone of the tank for each exposure in the habituation series.

Figure 12.

Figure 12—figure supplement 1. Additional data for novel tank habituation experiments.

Figure 12—figure supplement 1.

(A) The average number of freezing bouts per fish over the course of 6 exposures. (B) The number of fish that froze out of the total number of fish in the experiment. * indicates data for 3 fish lost from one exposure due to video equipment malfunction. These data could not be generated again since fish had already been exposed to the tank, so the group N for this exposure is 20 instead of 23.