| Gene (Danio rerio) |
klc4 |
Ensembl |
ENSDARG00000086985 |
|
| Strain, strain background (Danio rerio) |
AB |
ZIRC |
ZL1 |
Wildtype Strain of Zebrafish |
| Strain, strain background (Danio rerio) |
TL |
ZIRC |
ZL86 |
Wildtype Strain of Zebrafish |
| Genetic reagent (Danio rerio) |
Tg(–3.1ngn1:gfp-caax) |
Andersen et al., 2011
|
Tg(ngn:gfp-caax) |
Transgenic line labelling sensory axons, AB/TL background |
| Recombinant DNA reagent |
3.1ngn1:trfp-caax |
Andersen et al., 2011
|
Tg(ngn:trfp-caax) |
Plasmid for labelling sensory axons |
| Recombinant DNA reagent |
3.1ngn1-mcherry-caax |
Andersen et al., 2011
|
Tg(ngn:mcherry-caax) |
Plasmid for labelling sensory axons |
| Recombinant DNA reagent |
3.1ngn1:eb3-caax |
Stepanova et al., 2003; Ponomareva et al., 2014
|
Tg(ngn:eb3-gfp) |
Plasmid for labelling microtubule plus ends |
| Recombinant DNA reagent |
3.1ngn1:GFP-rab5c |
Clark et al., 2011; Ponomareva et al., 2014
|
Tg(ngn:GFP-rab5c) |
Plasmid for labelling Rab5c vesicles |
| Recombinant DNA reagent |
3.1ngn1:PA-gfp-Rab5c |
Ponomareva et al., 2014
|
Tg(ngn:PA-gfp-Rab5c) |
Plasmid for labelling Rab5c vesicles |
| Sequence-based reagent |
klc4 in situ probe |
This paper |
|
Sequences listed in methods |
| Sequence-based reagent |
klc4 long isoform specific in situ probe |
This paper |
|
Sequences listed in methods |
| Sequence-based reagent |
short isoform RT primers |
This paper |
|
Sequences listed in methods |
| Sequence-based reagent |
long isoform RT primers |
This paper |
|
Sequences listed in methods |
| Sequence-based reagent |
klc4 gRNA |
This paper |
|
Sequence listed in methods |
| Peptide, recombinant protein |
Cas9 protein with NLS |
PNA Bio |
NC0789474 |
|
| Chemical compound, drug |
Trizol |
Thermo-Fisher |
15596026 |
For RNA extraction |
| Chemical compound, drug |
Kinesore |
Chembridge Hit2Lead library |
#6233307 |
Kinesin1 targeting small molecule |
| Commercial assay or kit |
Superscript III |
Invitrogen |
18080093 |
|
| Commercial assay or kit |
DIG RNA labelling kit |
Roche |
11175025910 |
|
| Chemical compound, drug |
Oligo dT |
Invitrogen |
18418020 |
|
| Chemical compound, drug |
Random Hexamers |
Invitrogen |
N8080127 |
|
| Antibody |
anti-HNK1 (mouse monoclonal) |
ZIRC |
ZN-12 |
IF(1:250) |
| Antibody |
anti-acetylated tubulin (Mouse monoclonal) |
Sigma |
T6793 |
IF(1:1000) |
| Antibody |
anti-mouse IgG AlexaFluor 488 (goat polyclonal) |
Invitrogen |
A-21121 |
IF(1:1000) |
| Antibody |
Goat anti-mouse IgG2b AlexaFluor 647 (goat polyclonal) |
Invitrogen |
A-21141 |
IF(1:1000) |
| Software, algorithm |
CRISPRscan |
Moreno-Mateos et al., 2015
|
|
https://www.crisprscan.org/
|
| Software, algorithm |
CRISP-ID |
Dehairs et al., 2016
|
|
http://crispid.gbiomed.kuleuven.be/
|
| Software, algorithm |
ToxTrac |
Rodriguez et al., 2018
|
|
Animal Tracking Freeware |
| Software, algorithm |
OrientationJ |
Rezakhaniha et al., 2011
|
|
Used to quantify orientation of axons |
| Software, algorithm |
Graphpad Prism |
Graphpad |
|
Graph making software |
| Software, algorithm |
Chemotaxis Tool |
Ibidi |
|
https://ibidi.com/chemotaxis-analysis/171-chemotaxis-and-migration-tool.html
|
