TABLE 2.
Growth and/or survival of GBS strain 090 la LD
| Medium (mg/ml) | Mean CFU/ml (107) ± SD at time (h):
|
|||
|---|---|---|---|---|
| 0 | 1 | 3 | 5 | |
| Normal saline | 5.3 ± 0.9 | 5.9 ± 1.7 | 4.8 ± 1.3 | 4.0 ± 1.5 |
| Electrolyte solution | 5.2 ± 0.7 | 5.1 ± 0.8 | 6.4 ± 0.9 | 4.7 ± 0.6 |
| Nutrient-rich medium | ||||
| Without surfactant | 6.4 ± 0.6 | 15.2 ± 3.9 | 52.4 ± 11.4 | 59.3 ± 4.9 |
| With Curosurf (1) | 6.2 ± 2.0 | 13.5 ± 0.6 | 47.4 ± 7.8 | 66.1 ± 1.6 |
| With Curosurf (10) | 6.1 ± 1.6 | 14.8 ± 2.6 | 47.9 ± 4.0 | 58.8 ± 3.9 |
| With Curosurf (20) | 6.0 ± 0.8 | 14.2 ± 2.1 | 51.7 ± 6.7 | 59.8 ± 7.1 |
a
Growth and/or survival was measured in sterile saline in an electrolyte solution (28) mimicking alveolar fluid electrolyte content (sodium, 135 mmol/liter; chloride, 103 mmol/liter; potassium, 7.3 mmol/liter; calcium, 3.2 mmol/liter) and in a nutrient-rich medium (Standard I nutrient broth) containing 1, 10 or 20 mg of Curosurf per ml or no surfactant. Values are the mean CFU counts from four experiments.