Figure 6.

Downregulation of NOD2 caused activation of ERK that confers resistance to lenvatinib. (a) HepG2 and Huh7 cells were transfected as indicated for 24 h, then cells were treated with DMSO or SB202190 (20 μM) for another 24 h, and protein levels of NOD2 were measured. (b) HepG2 and Huh7 cells were transfected as indicated for 24 h, then cells were treated with DMSO or SCH772984 (20 μM) for another 24 h, and protein levels of NOD2 were measured. (c) HepG2 and Huh7 cells were transfected as indicated for 24 h, and indicated proteins were measured by western blots. (d) HepG2 and Huh7 cells were transfected as indicated for 24 h, and indicated proteins were measured by western blots. (e) HepG2 and Huh7 cells were treated with lenvatinib alone or in combination with SCH772984 (20 μM) for 24 h, and cellular death was measured. (f) Proposed model by which lncXIST regulates HCC cell response to lenvatinib.