Table 2.
Milk-clotting and proteolytic activities of proteases used in this study.
| Parameter | Recombinant camel chymosin | Chymosin from the calf stomach | Trompillo proteases1 |
|---|---|---|---|
| MCA, (SU mL−1) | 6120.2 ± 223.3a | 4397.4 ± 33.9b | 3658.8 ± 251.1c |
| Sp-MCA, (SU mg) protein−1 | 4697.2 ± 154.3b | 7064.7 ± 194.1a | 841.0 ± 55.2c |
| PA, (UTyr mL−1) | 32.6 ± 1.5b | 19.6 ± 0.5b | 535.6 ± 10.6a |
| Sp-PA, (UTyr mg) protein−1 | 24.8 ± 1.0c | 30.5 ± 0.8b | 50.2 ± 1.0a |
| Ratio MCA/PA | 187.7 ± 15.7b | 223.7 ± 8.4a | 6.8 ± 0.5c |
1Plant-derived protease from the fruits of Solanum elaeagnifolium. MCA=milk-clotting activity expressed in soxhlet units per milliliter (SU mL−1). Sp-MCA=specific milk-clotting activity expressed in soxhlet units per milligram of enzyme (SU mg−1). PA=proteolytic activity expressed in tyrosine units per milliliter (UTyr mL−1). Sp-PA=specific proteolytic activity expressed in tyrosin units per milligram of enzyme (UTyr mg−1). a,b,cMean values in the same row with different superscripts differ (ANOVA, Tukey-Kramer test, P < 0.05). Data represent the average of three replicates (n = 3).