Table 1. Primers used in real-time PCR.
Gene | Direction | Sequence | Size |
---|---|---|---|
GAPDH | Forward | 5′-CTCTGGCAAAGTCCAAGTGGTG-3′ | 103 bp |
Reverse | 5′-GCCCTTGAAGTGTCCGTGTGTA-3′ | ||
PRL | Forward | 5′-AAAGCTGTTAATGGCTGCCACAC-3′ | 122 bp |
Reverse | 5′-TCATTCCAGGAACGCAGCAC-3′ | ||
VIPR | Forward | 5′-GAGCTCGTGGTTGGGTCATTC-3′ | 87 bp |
Reverse | 5′-TCGCTTGAGCTCAGCTTGGA-3′ |
GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PRL, prolactin; VIPR, vasoactive intestinal peptide receptor 1.