Extended Data Fig. 6. A longer linker length does not impact neutralization potency of ReconnAbs.

(A) The linker used to tether ACE2 to either the C-terminus of the scFv or C-terminus of the COV2-2449 LC. The linker was designed to contain a Hexa-His tag for purification and a TEV site to facilitate proteolysis. (B) A longer linker length did not alter ReconnAb activity. One scFv (targeting either epitope A or B – as in Fig. 2f) was mutated to contain a linker that was 7aa longer than the previous study (B). An SDS-Page gel demonstrates that these proteins proteolyzed identically to those with the shorter linkers (C). The neutralization potency was not impacted by the increased linker length (D). Data shown is the average of 2 neutralization experiments.