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. 2022 Sep 8;18(11):1270–1276. doi: 10.1038/s41589-022-01140-1

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 7 — (A) The CV10-2449-ACE2-CrossMAb ReconnAb contains all designed components by SDS-PAGE, assayed before or after TEV cleavage and with or without 2-mercaptoethanol (BME). 1. Full-length ReconnAb, 2. Cleaved CrossMAb CV10-2449 IgG, 3. COV2-2449-LC-ACE2 fusion, 4. ACE2 (reduced ACE2 shows double banding), 5. HC, 6. Cleaved 2449-LC with linker, bottom band CV10 LC. (B) BLI binding of CV10-2449-ACE2-CrossMAb (brown) or the TEV cleaved form (teal) to SARS-CoV-2 spike. Binding is reduced upon TEV cleavage.