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. 2022 Oct 25;12:17835. doi: 10.1038/s41598-022-21850-9

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Differentiation potential and cell surface characterization of transduced and un-transduced hADSCs. Phase contrast microscopy (A1 and B1) images show fibroblast-like morphology of transduced hADSCs. For abiogenic and osteogenic differentiation, transduced hADSCs were stained with Oil red (B2, A2) and Alizarin-Red (A3, B3), respectively. Scale bar, 100 µm. For the immunophenotypic profile of the transduced hADSCs, the cells were stained with selected CD marker antibodies (positive for CD73 and CD105, negative for CD14/45) (C, D). All cells were selected from the 3rd to 5th passages.