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. 2022 Oct 25;12:17836. doi: 10.1038/s41598-022-20741-3

Table 2.

Common codes used to normalise the variation within EPG waveform nomenclature. All EPG waveform codes were coded to follow the standard aphid EPG codes. Psyllid EPG groupings were described in51 Chinch bug waveform descriptions followed52, Leafhopper characterisation53, planthopper characterisations54, whitefly characterisations55, and sharpshooter characterisation56. Note that for the sharpshooter studies xylem ingestion was coded as E2 to represent ingestion of the primary plant sap these insects feed from.

Insect group EPG waveform Plant tissue involved/insect behaviour Comparable aphid waveform (standardised definition)
Aphid Np phase None/insect on the plant but not probing plant tissue np
C phase Epidermal and mesophyll/insect probing plant tissue C
pd phase Mesophyll/puncture of plant cells by insect pd
F phase Mesophyll/stylet penetration difficulties F
G phase Xylem/ingestion of xylem sap G
E1 phase Phloem/salivation into phloem E1
E2 phase Phloem/ingestion of phloem E2
sE2 phase Phloem/sustained ingestion of phloem sE2
Chinch bug Z1 phase None/insect not on the plant np
Z2 phase None/insect on the plant but not probing plant tissue np
G1 phase Epidermal and mesophyll/insect probing plant tissue and secretion of saliva into mesophyll tissue C
G2 phase C
H phase Epidermal and mesophyll/insect probing plant tissue, start of penetration into vascular tissue C
N Phase Vascular/Salivation into a vascular cell N/A
J Phase Phloem/penetration of phloem sieve element and salivation into phloem E1
J-I1 Phase Phloem/ingestion of phloem sap mixed with salivation E1/E2
J-I2 Phase Phloem/ingestion of phloem sap E2
Leafhopper NP None/insect on the plant but not probing plant tissue np
R Epidermal and mesophyll/insect stylet is inserted into plant tissue but insect is at rest and not progressing penetration C
A Epidermal and mesophyll/insect begins probing of plant tissue C
C Epidermal and mesophyll/insect ingestion of mesophyll sap C
S Phloem/salivation into phloem E1
E Phloem/ingestion of phloem E2
F Phloem/difficult ingestion of phloem E2
Planthopper NP None/insect on the plant but not probing plant tissue np
N1, N2, N3 Epidermal and mesophyll/insect probing plant tissue C
N4-a Phloem/salivation into phloem E1
N4-b Phloem/ingestion of phloem E2
N5 Xylem/ingestion of xylem sap G
N6 Mesophyll/stylet penetration difficulties F
N7 Mesophyll/puncture of plant cells by insect pd
Psyllid NP None/insect on the plant but not probing plant tissue np
A Epidermal and mesophyll/insect penetration of plant tissue, sheath salivation C
B Epidermal and mesophyll/sheath salivation C
C Epidermal and mesophyll/continued sheath salivation and mesophyll probing C
D Mesophyll and phloem/putative salivation outside of phloem cell and putative contact with and salivation into phloem E1
E1 Phloem/salivation into phloem E1
E2 Phloem/ingestion of phloem E2
G Xylem/ingestion of xylem sap G
Whitefly np None/insect on the plant but not probing plant tissue np
A Epidermal and mesophyll/initial contact with plant tissue C
C Epidermal and mesophyll/insect probing plant tissue C
pd Mesophyll/puncture of plant cells by insect pd
E(pd1) Phloem/salivation into phloem E1
E(pd2) Phloem/ingestion of phloem E2
F Mesophyll/stylet penetration difficulties F
G Xylem/ingestion of xylem sap G
Sharpshooter Z Non-probing np
A1 Pathway C
B1 Pathway C
B2 Pathway C
C Ingestion E2
G Resting N/A
R Resting N/A
M Intoxication N/A
N Nonpathway interruption N/A

N/A denotes species-specific waveforms where there is no comparable aphid waveform.