Table 1.
Cell screening-imaging platforms.
| Comparison criteria | Arrayed screening8,48 | In situ sequencing24,25 | Photo-activation18,20,27,49 | CRaft-ID30 | Raft-seq(this paper) |
|---|---|---|---|---|---|
| a. Imaging constraints | No limitations | Impacted by seq chemistry | Limited by # of usable channels | Optics impacted by raft | Optics impacted by raft |
| b. How cells are Collected | NA | NA | FACS | Raft picking | Raft picking |
| c. Genotype resolution | Bulk | Single cell | Bulk | Colony | Single cell |
| d. Live cell collection | No | No | Medium viability | Possible | Yes |
| e. Genotyping method | NA | Specialized | Bulk NGS | Colony NGS | Single-cell NGS |
| f. Pooled scalability | Not pooled | Highest | Yes | Yes | Yes |
| g. Bottleneck | Physical plates | ISS Technology | Photo activation | Raft picking | Raft picking |