Table 2.

List of primers used in this study.

Primer Name	Sequence (5'–3')
T3_pET	ATAAGATCTAATTAACCCTCACTAAAGGGGAATTGTGAGCGGATAAC
SP6_pET	ATAAGATCTATTTAGGTGACACTATAGGGGAATTGTGAGCGGATAAC
T7_pET	ATAAGATCTTAATACGACTCACTATAGGGGAATTGTGAGCGGATAAC
ORF46P_pET	ATAAGATCTAAATACGCCAAGACTAGCGGGGAATTGTGAGCGGATAAC
ORF45P_pET	ATAAGATCTTCAGAAGTCACACTATAAGGGAATTGTGAGCGGATAAC
ORF53P_pET	ATAAGATCTAATAGAAACATACTATAAGGGAATTGTGAGCGGATAAC
ORF54P_pET	ATAAGATCTAAATAAGACTCGCTAAGGGGAATTGTGAGCGGATAAC
phiKDA1_1_pET	ATAAGATCTGATTAACGTAGAGGATAGGGGAATTGTGAGCGGATAAC
phiKDA1_2_pET	ATAAGATCTCCTCAGCCATAACTAAGGGGGAATTGTGAGCGGATAAC
phiKDA1_3_pET	ATAAGATCTTACAACACTATACAATAGGGGAATTGTGAGCGGATAAC
pET_rev	TCCGGATATAGTTCCTC
Promoter ORF45 200 bp fusion fw	TCAGAAGTCACACTATAAGGCGTAAATTATGGGATTTTACCA
Promoter T7 200 bp fusion fw	TAATACGACTCACTATAGGGCGTAAATTATGGGATTTTACCA
200 bp rv	TTGCAGTTGTGGTGTATTTTAATCT

Underlined sequences are candidate promoters integrated into primers for template DNA preparation for transcription. All primers were synthesized at Eurofins MWG Operon (Ebersberg, Germany).