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. 2022 Jun 24;61(8):3887–3903. doi: 10.1007/s00394-022-02928-6

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — RDA plots showing factors that significantly explain the variance in the microbiota composition and the loadings for each component of RDA1 and RDA2 (stepwise-built Model 4 shown in Supplementary resource 4). Study participants’ weighted scores are represented by black-filled circles (WDP) and empty circles (HDP). Crosses show scores for genera. Thick black arrows represent explanatory variables, while thin grey arrows represent genera. Genera that were weakly associated with the first two axes are omitted for clarity. Loadings for RDA1 and RDA2 axes are shown below. Bars for the genera with significantly higher relative abundance in people with WDP and HDP (LEfSe result) have been indicated with black-filled and white-filled arrows, respectively (thicker arrows correspond to bacteria where log₁₀(LDA score) > 2.0, thinner lines to bacteria where 1.8 < log₁₀(LDA score) < 2.0, whereas dashed arrows to bacteria differentially abundant shown only in Mann–Whitney U-test)