Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 25;79(11):565. doi: 10.1007/s00018-022-04585-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — mitoKeima detection of Gp78-dependent basal mitophagy in HT-1080 cells. Wild-type HT-1080 cells and g2-41 knockout CRISPR clones were transfected with mito-Keima and treated with DMSO or CCCP for 24 h. Confocal fluorescence images show mito-Keima in both neutral (yellow) and low pH (red) environments. Accumulation of mitolysosomes (red) is observed selectively in HT-1080 cells and increased upon CCCP treatment. Quantification of both the number of mitolysosomes per cell and the total area of mitolysosomes per cell is shown in the bar graphs (Scale bar: 10 μm; n = 3 independent biological replicates; > 10 cells/condition per experiment; *, p < 0.05; **, p < 0.01; ***, p < 0.001; ns, no significance; Mean ± SEM)