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. 2022 Oct 25;12:17872. doi: 10.1038/s41598-022-21522-8

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PCR strategies to detect tandem gene duplication. (A) PCR with inverted (outward facing) primer pair. Successful PCR-amplification indicates presence of direct or inverted tandem duplication. (B) PCR with single primer (Single Primer PCR): Successful PCR amplification indicates presence of inverted tandem duplication. Harpoon represents primers, solid red arrow bar represents a genomic locus and black horizontal line represents PCR amplicon.