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. 2022 Oct 25;12:17856. doi: 10.1038/s41598-022-22507-3

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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HTR-8 cell migration is regulated by ligands targeting CRY1/2 and NR1D1/2. Wound healing assays in HTR-8 cells, show that (A, B) KL001, a drug stabilizing CRY1/2, but not KL101, a drug stabilizing CRY1, reduced HTR-8 cell migration. (C, D) PF670462, a drug preventing PER1/2/3 degradation, did not impact HTR-8 cell migration. (E–G) The NR1D1/2 agonist SR9009, had no significant effect on migration, whereas the NR1D1/2 antagonist/inverse agonist, SR8278, decreased HTR-8 cell migration. All drugs were tested at 1 and 10 µM. The data were pooled if the two doses were not significantly different. (B, D, F, G) Illustrative wound healing assay images. Dotted line on histograms represents approximate wound area in control at 24 h. Data is expressed as % change in average wound size from 0 h ± SEM. N = 3–6, in duplicate. One-way ANOVA repeated measures, *, p < 0.05; ***, p < 0.001.