Table 1.
Descriptive statistics of 17 candidate circadian clock genes’ expression levels in placenta from PE vs. non-PE, independent of gestation length (overall PE vs overall non-PE, respectively), in two independent datasets.
| Gene | GSE75010-157 samples | GSE75010-173 samples | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Non-PE | PE | pb | FDRc | Non-PE | PE | p | FDR | |||||
| n | Meana (SD) | n | Mean (SD) | n | Meana (SD) | n | Mean (SD) | |||||
| ARNTL | 77 | 7.36 (0.38) | 80 | 7.38 (0.42) | 0.7006 | 0.8507 | 96 | 7.38 (0.39) | 77 | 7.35 (0.32) | 0.5509 | 0.7128 |
| ARNTL2 | 77 | 7.95 (0.40) | 80 | 8.09 (0.42) | 0.0346 | 0.0735 | 96 | 8.01 (0.41) | 77 | 8.01 (0.43) | 0.9995 | 0.9995 |
| CLOCK | 77 | 8.88 (0.50) | 80 | 8.73 (0.53) | 0.0766 | 0.1302 | 96 | 8.88 (0.53) | 77 | 8.71 (0.40) | 0.0181 | 0.0711 |
| CRY1 | 77 | 8.39 (0.41) | 80 | 8.53 (0.39) | 0.0250 | 0.0607 | 96 | 8.40 (0.40) | 77 | 8.53 (0.36) | 0.0209 | 0.0711 |
| CRY2 | 77 | 8.39 (0.27) | 80 | 8.38 (0.28) | 0.9098 | 0.9098 | 96 | 8.40 (0.29) | 77 | 8.38 (0.26) | 0.6289 | 0.7128 |
| NPAS1 | 77 | 6.56 (0.36) | 80 | 6.76 (0.39) | 0.0008 | 0.0045 | 96 | 6.68 (0.44) | 77 | 6.61 (0.45) | 0.2943 | 0.5440 |
| NPAS2 | 77 | 9.06 (0.37) | 80 | 8.96 (0.32) | 0.0759 | 0.1302 | 96 | 8.99 (0.38) | 77 | 9.03 (0.32) | 0.4382 | 0.6607 |
| NPAS3 | 77 | 6.55 (0.42) | 80 | 6.45 (0.37) | 0.1226 | 0.1788 | 96 | 6.51 (0.40) | 77 | 6.48 (0.39) | 0.6276 | 0.7128 |
| NR1D1 | 77 | 7.23 (0.40) | 80 | 7.32 (0.34) | 0.1262 | 0.1778 | 96 | 7.28 (0.36) | 77 | 7.22 (0.41) | 0.2638 | 0.5440 |
| NR1D2 | 77 | 8.80 (0.48) | 80 | 8.45 (0.51) | < 0.0001 | 0.0008 | 96 | 8.70 (0.51) | 77 | 8.49 (0.41) | 0.0030 | 0.0255 |
| PER1 | 77 | 7.40 (0.37) | 80 | 7.41 (0.47) | 0.8812 | 0.9098 | 96 | 7.37 (0.44) | 77 | 7.43 (0.39) | 0.3200 | 0.5440 |
| PER2 | 77 | 7.93 (0.38) | 80 | 8.08 (0.32) | 0.0096 | 0.0272 | 96 | 8.05 (0.33) | 77 | 7.95 (0.36) | 0.0737 | 0.2088 |
| PER3 | 77 | 7.30 (0.32) | 80 | 7.04 (0.30) | < 0.0001 | 0.0008 | 96 | 7.25 (0.30) | 77 | 7.03 (0.27) | < 0.0001 | 0.0017 |
| RORA | 77 | 8.54 (0.43) | 80 | 8.52 (0.40) | 0.7598 | 0.8611 | 96 | 8.59 (0.42) | 77 | 8.45 (0.36) | 0.0204 | 0.0711 |
| RORB | 77 | 6.64 (0.67) | 80 | 7.00 (0.93) | 0.0063 | 0.0214 | 96 | 6.79 (0.67) | 77 | 6.87 (0.82) | 0.4664 | 0.6607 |
| RORC | 77 | 6.56 (0.35) | 80 | 6.64 (0.35) | 0.1711 | 0.2237 | 96 | 6.55 (0.39) | 77 | 6.62 (0.38) | 0.2341 | 0.5440 |
| TIMELESS | 77 | 7.92 (0.40) | 80 | 7.73 (0.32) | 0.0015 | 0.0064 | 96 | 7.82 (0.37) | 77 | 7.83 (0.36) | 0.9138 | 0.9709 |
aMean of gene expression values, normalized and log2-transformed. The mean change percentages of 3 circadian genes’ expression levels are: for CRY1 gene, (8.53–8.39)*100/8.39 = 1.7% in GSE75010-157 Samples and (8.53–8.40)*100/8.40 = 1.5% in GSE75010-173 Samples; for NR1D2 gene, (8.80–8.45)*100/8.80 = 4.0% in GSE75010-157 Samples and (8.70–8.49)*100/8.70 = 2.4% in GSE75010-173 Samples; and for PER3 gene, (7.30–7.04)*100/7.30 = 3.6% in GSE75010-157 Samples and (7.25–7.03)*100/7.25 = 3.0% in GSE75010-173 Samples.
bTwo-sample, two-tailed t-tests were used to generate p-values.
Bold values denote statistical significance (p < 0.05 and FDR < 0.10) in both datasets.