Table 3.
List of IF Primary and Secondary Antibodies
| Antibodies | Supplier | Catalog number | Concentration |
|---|---|---|---|
| Primary antibodies | |||
| Anti-human IL22 | Cloud-Clone Corp | MAC032Hu22 | 1:100 |
| Anti-human CD66b | Novus Biotechnologies | G10F5 | 1:100 |
| Anti-mouse IL22 | Santa Cruz Biotechnology | SC-14436 | 1:100 |
| Anti-mouse Ly6G | BioLegend | 127602 | 1:50 |
| Anti-mouse CD3 | Abcam | Ab16669 | 1:100 |
| Anti-human/mouse MPO | R&D | AF3667 | 1:100 |
| Anti-mouse F4/80 | Synaptic System, Germany |
397004 | 1:50 |
| Anti–mouse–α-SMA | Sigma-Aldrich | A2547 | 1:100 |
| Anti-mouse desmin | Thermo Fisher Scientific, Massachusetts, US | PA5-1670 | 1:100 |
| Secondary antibodies | |||
| Donkey anti-mouse CF568 | Sigma-Aldrich | SAB4600315 | 1:500 |
| Donkey anti-mouse A647 | Thermo Fisher Scientific | A31571 | 1:500 |
| Donkey anti-mouse IgM A488 | Jackson ImmunoResearch | 715-545-020 | 1:300 |
| Donkey anti-goat A568 | Thermo Fisher Scientific | A11057 | 1:500 |
| Chicken anti-rat A678 | Thermo Fisher Scientific | A21472 | 1:250 |
| Donkey anti-rabbit A678 | Thermo Fisher Scientific | A31573 | 1:500 |
| Donkey anti-rabbit A488 | Thermo Fisher Scientific | A21206 | 1:500 |
| Donkey anti- guinea pig A678 | Jackson ImmunoResearch | 706-605-148 | 1:500 |
α-SMA, α-smooth muscle actin.