Fig. 11.

Arg483 mutation weaks the protective effect of quercetin on atherosclerosis in vivo.

AAV-KEAP1-WT and AAV-KEAP1-R483S were injected intravenously through the tail of the mouse into 8-weeks-old ApoE^−/− mice, all of which were given Quer diet. (A) Representative images for HE, Masson, and Oil red O stain. (B). Quantized data for HE staining (n = 3, *p < 0.05). (C). Quantized data for Oil red stain (n = 3, *p < 0.05). (D). Quantized data for Masson stain (n = 3, *p < 0.05). (E, G). NLRP3 and Mac-3 co-staining in aortic sinus (n = 3, **p < 0.01). (F, H). Caspase1 and Mac-3 co-staining in aortic sinus (n = 3, ***p < 0.001). (I, J) Western blot is used to evaluate NLRP3, Cle-caspase1, N-GSDMD, NRF2, and HO-1 levels (n = 5). **p < 0.01, ***p < 0.001. (K, L) The serum level of IL-18 and IL-1β (n = 5). **p < 0.01. . (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)