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. 2022 Oct 14;57:102511. doi: 10.1016/j.redox.2022.102511

Fig. 3.

Fig. 3

Quercetin suppresses the pyroptosis of macrophage induced by ox-LDL.

THP-1 macrophages are pretreated with quercetin (50 μM) for 1 h, then stimulated with 100 μg/ml ox-LDL for 24 h. Scanning electron microscope, Hochest/PI staining, CCK-8 and LDH assay are used to evaluate the shape change and cell viability of macrophages in different groups, western blot is used to analyze the protein level of NLRP3 inflammasome. (A, B). Representative images for scanning electron microscope and the diameter of macrophage in different groups (n = 3 control vs n = 3 ox-LDL, **p < 0.01. n = 3 ox-LDL vs n = 3 ox-LDL + Quer, #p < 0.05). (C, D). Representative images and quantification of Hoechst/PI staining (n = 3 control vs n = 3 ox-LDL, ***p < 0.001. n = 3 ox-LDL vs n = 3 ox-LDL + Quer, ###p < 0.001). (E). CCK-8 (n = 3 control vs n = 3 ox-LDL, **p < 0.01. n = 3 ox-LDL vs n = 3 ox-LDL + Quer, #p < 0.05). (F). LDH assay (n = 3 control vs n = 3 ox-LDL, **p < 0.01. n = 3 ox-LDL vs n = 3 ox-LDL + Quer, #p < 0.05). (G, H). The NLRP3, N-GSDMD, cle-caspase1, IL-18, and IL-1β protein levels (n = 3, control vs ox-LDL group, *p < 0.05, **p < 0.01. n = 3 ox-LDL vs ox-LDL + Quer group, #p < 0.05).