Table 3.

Gene expression profile of AFSCs

Author, year (Ref)	Markers	Antigen or CD	First trimester	Second and third trimester
	Oct4	+ +
	Nanog	+ ${}^{\_}$
	Sox2	+ ${}^{\_}$
	SSEA4	+ +
	c-Myc	+ +
	Tra-1-60	+ ${}^{\_}$
	SSEA3	+ ${}^{\_}$
	Tra-1-81	+ ${}^{\_}$
	ALPL	+ ${}^{\_}$
Loukogeorgakis et al. , 2017	Stem cell	Klf4	+ ${}^{\_}$
Cananzi et al. , 2009		CD29	+ +
Guillot et al. , 2007		CD44	+ +
Baghaban Eslaminejad et al. , 2012 (59-62)		CD73	+ +
		CD90	+ +
		CD105	+ +
		CD166	+ +
		Mesenchymal	CD146	+ ${}^{+}$
			CD45	${}^{\_}$	${}^{\_}$
			CD34	${}^{\_}$	${}^{\_}$
	CD31		${}^{\_}$	${}^{\_}$
	Hematopoietic		CD14	${}^{\_}$	${}^{\_}$
			HLA class I	${}^{+}$	${}^{+}$
			MHC	HLA class II	${}^{\_}$	${}^{\_}$
AFSCs: Amniotic fluid stem cells, CD: Cluster of differentiation, Oct4: Octamer-binding transcription factor 4, Nanog: Homeobox transcription factor nanog, Sox2: Sex determining region Y-box 2, SSEA4: Stage-specific embryonic antigen-4, c-Myc: A family of regulator genes and proto-oncogenes that code for transcription factors, Tra-1-60: A cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, SSEA3: Stage-specific embryonic antigen 3, Tra-1-81: A cell surface antigen expressed along with SSEA-3, SSEA-4 and TRA-1-60, ALPL: Provides instructions for making an enzyme called tissue-nonspecific alkaline phosphatase, Klf4: Kruppel like factor 4, MHC: Major histocompatibility complex, HLA: Human leucocyte antigen