Figure 4.

POLE EDMs promoted cell migration and invasion of endometrial cancer cells. HEC-1A cells were transiently transfected with wildtype POLE or P286R, R375Q, V411L, or P452L variant. (A) Transwell analysis was performed to examine cell invasion. (B) Wound healing assay was conducted to examine cell migration. (C) Western blot was carried out at 48 h after transfection to measure the protein levels of N-cadherin, E-cadherin, Slug, and Snail. β-actin was used as an internal reference. Data are expressed as the mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001, vs. WT; n = 3. WT, wildtype.