Table 3.
Primers sequences of target genes selected for analysis by real-time.
| Gene | Primer sequences (5′-3′) | Size, bp | Temperature, °C |
|---|---|---|---|
| TNF-α | F: ACCTCCTCTCTGCCATCAAG R: CTGCCCAGATTCAGCAAAGT |
173 | 60 |
| IL-1β | F: ATTCAGGGACCCTACCCTCTC R: ATCACTTCCTTGGCGGGTTC |
92 | 60 |
| IL-1α | F: GCATGTGCTGAGCCTTTGTA R: CCTGGTCCTCCCAAGATTGT |
181 | 60 |
| IL-6 | F: GATGCTTCCAATCTGGGTTCA | 62 | 58 |
| R: CACAAGACCGGTGGTGATTC | |||
| IL-10 | F: CGGCCCAGTGAAGAGTTTCT | 98 | 60 |
| R: GGCAACCCAGGTAACCCTTA | |||
| SOD | F: GAGACCTGGGCAATGTGACT | 118 | 60 |
| R: CTGCCCAAGTCATCTGGTTT | |||
| GCLC | F: CAAACCATCCTACCCTTTGG | 172 | 58 |
| R: ATTGTGCAGAGAGCCTGGTT | |||
| GCLM | F: GATGCCGCCCGATTTAACTG | 159 | 58 |
| R: ACAATGACCGAGTACCGCAG | |||
| HO-1 | F: CGCTCCCGAATGAACACTCT | 148 | 60 |
| R: GCGAGGGTCTCTGGTCCTTA | |||
| NQO-1 | F: ATCACAGGTAAACTGAAGGACCC | 229 | 60 |
| R: TGGCAGCGTATGTGTAAGCA | |||
| β-Actin | F: TCTGGCACCACACCTTCT R: TGATCTGGGTCATCTTCTCAC |
114 | 57 |
TNF-α = tumor necrosis factor-α; IL = interleukin; SOD = superoxide dismutase; GCLC = glutamate cysteine ligase catalytic subunit; GCLM = glutamate-cysteine ligase regulatory subunit; HO-1 = heme oxygenase-1; NQO-1 = phosphate adenine dinucleotide quinone oxidoreductase-1.