Figure 4.

Knockdown of miR-143 antagonizing the EGCG regulation of expression levels of miR-143 and MAPK7, as well as cell proliferation in 3T3-L1 preadipocytes. The cells are pretreated with miR-143 inhibitor or negative control (NC) for 24 h and followed by 50 μM EGCG treatment for 48 h. (a) miR-143 expression was analyzed via qPCR. (b) MAPK7 expression was analyzed by qPCR. (c) The protein level of MAPK7 was measured by western blot. (d) Cell number. Data are presented as mean ± SEM (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001, compared with the NC group. ^#P < 0.05, ^##P < 0.01, EGCG + NC versus EGCG + miR-143 inhibitor. (A color version of this figure is available in the online journal.)