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. 2022 Jun 22;28(4):827–840. doi: 10.3350/cmh.2022.0068

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Copyright © 2022 by The Korean Association for the Study of the Liver

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 2. — Auranofin reduces the fibrosis marker and cell proliferation of TGF-β1 induced LX-2 cells. (A) Quantitative comparison of fibrosis marker mRNA levels and (B) protein expression. Data shown in bar diagrams as mean±standard error of mean from three independent experiments. (C) Immunofluorescence staining of LX-2 cells with collagen1 (green) was shown as a confocal image (×630). (D) Wound healing assay using auranofin on TGF-β1-induced LX-2 cells (×100). TGF, transforming growth factor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; COL1A1, collagen type 1 alpha 1 chain; PDGF-A, platelet-derived growth factor A. ^*P<0.05. ^**P<0.01. ^***P<0.001. ^****P<0.0001.