Figure 4.

Auranofin reduces intrahepatic fibrosis in the BDL model. (A) Experimental schematic and (B) day 21 mice images in BDL model mice. (C) H&E, Sirius red staining and immunohistochemical images of FN-1 in a liver sample from BDL model mice (×100). (D) Quantitative comparison of fibrosis area based on Sirius red staining. (E) Hepatic α-SMA and FN-1 mRNA and (F) NF-κB protein expression in BDL model mice. (G) The survival rate of BDL model mice (vehicle, n=8; AR 1 mg, n=11; AR 3 mg, n=11; and AR 10 mg, n=12). H&E, hematoxylin and eosin; FN-1, fibronectin 1; NF-κB, nuclear factor kappa B; BDL, bile duct ligation. ^*P<0.05. ^**P<0.01.