TABLE 1.

Overview of open-source software packages developed for mother machine segmentation and lineage tracking. The applicability to specific imaging modalities indicates which ones have been demonstrated. All programs are available on Github or Gitlab.

Software	Language	Deep learning based	Phase contrast segmentation	Brightfield segmentation	Fluorescence segmentation
Molyso (Sachs et al., 2016)	Python		✓
MoMA (Kaiser et al., 2018)	Java		✓		✓
MMHelper (Smith et al., 2019)	Python		✓	✓
BACMANN (Ollion et al., 2019)	Java		✓		✓
DeLTA (Lugagne et al., 2020; O’Connor et al., 2022)	Python	✓	✓
DistNET (Ollion and Ollion, 2020)	Python	✓	✓