Figure 5.

Pseudotime analysis of transcription factor expression during human embryonic stem cell-derived endothelial cells differentiation. (A) Differential gene expression variance over pseudotime of H9 differentiation within pseudotime trajectory branches. Genes used are TF that are differentially expressed in both H9 and RC11-derived trajectories. (B) Gene expression variance of TF taken from Cluster 5 of heatmap from A in the endothelial branch of the trajectory from the five-factor protocol. (C) Spline plots showing the expression of novel TF from B across pseudotime in RC11 and H9 human embryonic stem cell-derived endothelial cells differentiation using the original protocol, as well as for the five-factor protocol.