NSF increased muscle strength, size, and mass in old mice. Measurements of (A) body weight and (B) grip strength of mice given NSF for 6 weeks. (C) Representative images of the gastrocnemius (GAS, top) and quadriceps (QUA, bottom) muscles. (D) The muscle mass of GAS (left) and QUA (right). Muscle mass was standardized to the body weight obtained on the last day. Ctrl, control; YM, young mice. The comparisons were performed using one-way ANOVA followed by Tukey's post-hoc test. Distinct letters present statistically considerable differences; p < 0.05, a > ab > b > bc > c > d.