Fig. 4.

NSF regulated inflammatory responses that affect sarcopenia in old mice. (A) Serum levels of pro-inflammatory cytokines (interleukin (IL)-1, IL-6, and tumor necrosis factor α (TNF-α) and C-reactive protein (CRP). (B) Weights of the spleen. Spleen weights were standardized to the body weight obtained on the last day. (C) Total number of splenocytes. (D) Gating scheme for macrophages (top left). The red particles present the isotype controls and black particles display the cells dyed with particular antibodies. The bar graph shows the mean percentage of CD11b⁺F4/80⁺ macrophages in splenocytes (top right). Representative dot plots of CD11b⁺F4/80⁺ macrophages (bottom). YM, young mice. The comparisons were performed using one-way ANOVA followed by Tukey's post-hoc test. Distinct letters present statistically considerable differences; p < 0.05, a > ab > b > c.