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. 2022 Oct 12;13:1000819. doi: 10.3389/fpls.2022.1000819

Figure 3.

Figure 3

Identification of the AaBPS coding sequence and AaBPS expression. (A) Camphor biosynthesis pathway modified from (Croteau et al., 1981). (B) Expression of AaBPS and AaBPS-likeA in L1-5 juvenile, L7-9 expanding and L11-13 mature leaves. Error bars – SE (n=12). nd- transcript not detectable by qRT-PCR. * - statistically significant difference (t-test) between Artemis and camphor-0 at p<0.05. (C) Predicted AaBPS protein sequence was modelled (green) using the I-TASSER approach (C-score 0.5) and overlaid on sage (purple) Bornyl-diPhosphate Synthase (SoBPS, pdb structure 1N1B, 40% amino acid identity) I-TASSER predicted GPP binding residues highlighted in yellow, predicted catalytic W326 in red. Mg2+ (green) and Hg2+ (grey) ions from 1N1B structure are also shown.