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. 2022 Oct 12;13:1000819. doi: 10.3389/fpls.2022.1000819

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Copyright © 2022 Czechowski, Branigan, Rae, Rathbone, Larson, Harvey, Catania, Zhang, Li, Salmon, Bowles, O´Maille and Graham

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Genomic deletion in camphor-0 lines. (A) Location of primers (P1-P8) designed for various parts of AaBPS gene including5’ promoter region (Primer sequences in Table S3 ). (B) PCR amplification on genomic DNA isolated from 14 camphor-0 M2 (lines 1-14), two individual Artemis F1 (lines 15-16), Artemis Parents C4 (line 17) and C1 (line 18) using primers annotated on panel (A) Line 19 – no template control. Line M – Gene Ruler™ 1kb DNA ladder (Thermo Fisher). Amplification of full length AaAMS gene (GenBank Accession AF327527) used as a positive control. Size of PCR amplicons predicted from genomic DNA sequence shown.