Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 12;13:1000819. doi: 10.3389/fpls.2022.1000819

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2022 Czechowski, Branigan, Rae, Rathbone, Larson, Harvey, Catania, Zhang, Li, Salmon, Bowles, O´Maille and Graham

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Functional characterisation of AaBPS. (A) Alignment of predicted amino acid sequence of AaBPS (GenBank accession OL656813) with SoBPS (GenBank accession AF051900). Truncation points used to generate truncated versions of AaBPS are indicated as BPS-tr1 and BPS-tr2. BPS-fl represents a full length protein. Red bars – active site lid, Blue bars - active site, Brown bar – substrate binding site annotated from Whittington et al., 2002. Identical (Black) and similar (grey) positions highlighted. (B) AaBPS in vitro protein activity assay using gas chromatography–mass spectrometry (GC-MS) based detection of monoterpenes. No protein control includes geraniol, the product of GPP hydrolysis (peak 1). Identification of peak 1 and 2 as geraniol and borneol respectively was assigned using known standards. Identities of peaks 3-10 were assigned using the NIST database and shown in (C). (C) Relative abundance of AaBPS in-vitro products for the most active truncated version of the protein (BPS-tr1). (D) Relative abundance of selected volatiles in three types of A annua Artemis leaves measured by GC-MS: L1-5 (juvenile), L7-9 (expanding), L11-13 (mature). Error bars – SE (n=6).