Fig. 1.

Sequence of the Fc-binding SAP fusion peptide pG_EAK. (a) The polypeptide is consisting of three Fc-binding domains in C1, C2, and C3 separated by D1 and D2 sequences and amphiphilic EAK repeats; (b) Congo red spectra of PBS solutions containing EAK, pG_EAK, a mixture of EAK and pG_EAK, and solution containing only the dye, showing peak intensities at 510 nm, 486 nm, and 506 nm, and 485 nm, respectively; (c) SDS-PAGE electrophoresis of unincorporated fraction of pG_EAK in the mixing; the fractions were quantified using ImageJ profile plot as indicated on the band with mixing ratio at 50:1, 100:1, 200:1, and 400:1 for EAK to pG_EAK; the numbers indicate the fraction of pG_EAK not incorporated into the fibrils; (d) viscosity profiles of solutions of PBS containing only EAK (1.5 mg/ml; red downward triangle), only pG_EAK (2.3 μM; green diamond), and EAK intermixed with pG_EAK (400:1; blue upward triangle) using μVISC (Rheosense). (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)