TABLE 5.
Test results of HIV-1 seroconversion serum panels by various methods (panels 16 to 19)
| HIV-1 seroconversion serum panel no. | Days after initial blood collection | Cutoff indexes
|
Maximal dilution to cause gelatin particle agglutination | Western blot
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| p24 antigen by previous immunoassay | p24 antigen by improved immunoassay | RNA | Anti-p17 IgG by previous immunoassay | Anti-p17 IgG by improved immunoassay | Anti-p17 IgM | Anti-HIV-1 antibodies by conventional EIA | gp160 | gp120 | gp41 | p24 | p17 | |||
| 16 (panel X) | 0 | 0.0 | 0.2 | <0.1 | 0.4 | 12 | 20 | − | − | − | − | − | ||
| 2 | 0.1 | 0.2 | <0.1 | 0.4 | 21 | 23 | − | − | − | − | − | |||
| 8 | 0.5 | 0.3 | <0.1 | 0.4 | 26 | 16 | − | − | − | − | − | |||
| 10 | 0.1 | 0.2 | <0.1 | 0.6 | 20 | 28 | − | − | − | − | − | |||
| 26 | 128 | 2,635 | 336 | 0.2 | 8.9 | 15 | − | − | − | − | − | |||
| 33 | 24 | 592 | 80 | 24 | 9,387 | 4,128 | 8 | − | − | − | − | − | ||
| 35 | 6.0 | 103 | 30 | 91 | 36,165 | 11,197 | 16 | − | − | − | + | − | ||
| 40 | 5.8 | 118 | 13 | 881 | 64,908 | 20,371 | 16 | + | − | − | + | − | ||
| 17 (panel Y) | 0 | 0.3 | 0.4 | <0.1 | 0.1 | 1.4 | 0.2 | − | − | − | − | − | ||
| 10 | 0.2 | 0.2 | <0.1 | 0.1 | 1.3 | 0.2 | − | − | − | − | − | |||
| 18 | 0.0 | 0.2 | <0.1 | 0.2 | 0.8 | 0.2 | − | − | − | − | − | |||
| 22 | 0.3 | 0.2 | <0.1 | 0.1 | 1.2 | 0.1 | − | − | − | − | − | |||
| 44 | 42 | 354 | 393 | 0.3 | 953 | 859 | 2 | − | − | − | + | − | ||
| 49 | 23 | 162 | 427 | 32 | 5,013 | 1350 | 8 | − | − | − | + | − | ||
| 18 (panel AR) | 0 | 0.1 | 0.4 | <0.1 | 0.1 | 6.3 | 0.5 | − | − | − | − | − | ||
| 2 | 0.1 | 0.3 | <0.1 | 0.2 | 5.2 | 0.6 | − | − | − | − | − | |||
| 9 | 0.4 | 9.1 | 25 | 0.0 | 7.3 | 0.6 | − | − | − | − | − | |||
| 14 | 47 | 776 | 2,200 | 0.1 | 11 | 0.5 | − | − | − | + | − | |||
| 19 (panel 6240) | 0 | 0.3 | 0.2 | <0.1 | 0.1 | 27 | 1.7 | − | − | − | − | − | ||
| 2 | 0.1 | 0.2 | <0.1 | 0.1 | 19 | 0.0 | − | − | − | − | − | |||
| 7 | 0.1 | 0.1 | <0.1 | 0.1 | 13 | 0.3 | − | − | − | − | − | |||
| 9 | 0.0 | 0.1 | <0.1 | 0.1 | 12 | 0.1 | − | − | − | − | − | |||
| 14 | 0.3 | 0.1 | <0.1 | 0.6 | 7.2 | 0.1 | − | − | − | − | − | |||
| 16 | 0.3 | 0.3 | <0.1 | 0.1 | 8.1 | 6.5 | − | − | − | − | − | |||
| 21 | 2.8 | 25 | 46 | 0.1 | 12 | 0.2 | − | − | − | − | − | |||
| 23 | 21 | 311 | 377 | 0.3 | 5.1 | 0.2 | − | − | − | − | − | |||
| 28 | 459 | 5,186 | 2,510 | 0.1 | 2.1 | 0.2 | − | − | − | − | − | |||
| 30 | 1,443 | 7,859 | 2,480 | 0.2 | 18 | 9.5 | − | − | − | + | − | |||
| 36 | 102 | 1,071 | 723 | 87 | 2,815 | 1,435 | 16 | + | − | − | + | − | ||
| 45 | 11 | 182 | 64 | 3,483 | 179,104 | 18,909 | 32 | + | − | − | + | − | ||
| 53 | 5.1 | 99 | 47 | 3,920 | 146,818 | 11,962 | 64 | + | − | − | + | − | ||
| Cutoff values | 0.9 | 0.5 | 400 | 1.9 | 0.7 | 1.5 | 0.1 | |||||||
| Signalsa | ||||||||||||||
| Mean | 0.5 | 0.1 | 0.7 | 0.2 | 0.2 | |||||||||
| SD | 0.1 | 0.1 | 0.4 | 0.1 | 0.2 | |||||||||
| Range | 0.1–0.9 | 0.1–0.5 | 0.1–1.9 | 0.1–0.7 | 0.1–1.5 | |||||||||
a
The signals are the fluorescence intensities of bound β-d-galactosidase activities.