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Relative expression levels of melanocortin-related genes in zebrafish ovaries and testes. Expression of the 18s rRNA gene was used as the reference gene for normalization of cDNA levels. Asterisk indicates significant variation after a two-way ANOVA analysis followed by Sidak’s multiple comparison test (*** p < 0.001, **** p < 0.0001). Data are expressed as mean ± SEM.
