Figure 3.

Depletion of KDM4A/B/C increases trimethylation of H3K9 leading to a decrease in HIF-1α in intermittent hypoxia. MCF7 cells were transfected with combined siKDM4A, siKDM4B, and siKDM4C (referred to as siKDM4) or siCONTROL followed by exposure to normoxia, chronic hypoxia (2% v/v), and intermittent hypoxia (5 min/5 min) over 18 h. mRNA expression of (A) KDM4A, (B) KDM4B, (C) KDM4C, (E) HIF1A, (F) HK2, and (G) PLOD2. All values are normalized to normoxia (Log2 scale). Results are the mean ± SD n ≥ 4 independent experiments. ns = not significant, ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. D, nuclear extracts of KDM4A, KDM4B, KDM4C, HIF-1α, H3K9me3, and histone H3 (loading control), and cytoplasmic extracts of HK2 and β-actin (loading control).