Table 2.
Nucleotide sequences and sizes of PCR products of amplified genes
| Gene | Oligonucleotide sequence (5′-3′) | Amplicon size (bp) | PCR conditions | Reference |
|---|---|---|---|---|
| mecA | AAAATCGATGGTAAAGGTTGGC AGTTCTGGCACTACCGGATTTGC | 533 | 94℃, 5 min, 40 cycles of 94℃ for 1 min, 58℃ for 1 min, 72℃ for 2 min, final extension 72℃ for 5 min | (23) |
| mecC | GAA AAA AAG GCT TAG AAC GCC TC GAA GAT CTT TTC CGT TTT CAG C | 138 | 94℃, 15 min, 30 cycles of 94℃ for 30 s, 59℃ for 1 min, 72℃ for 1 min, final extension for 10 min | (35) |
| blaZ ermA | ACTTCAACACCTGCTGCTTTC TAGGTTCAGATTGGCCCTTAG TCT AAAAAG CATGTAAAAGAA TGA TTATAATTATTT GATAGC TTC | 173 645 | 95℃, 3 min, 30 cycles of 95℃ for 30 s, 54℃ for 30 s, 72℃ for 30 s, final extension 72℃ for 4 min | (7) |
| ermB | TAACGACGAAACTGGCTAAAA ATCTGTGGTATGGCGGGTAAG | 416 | 95℃, 3 min, 30 cycles of 95℃ for 30 s, 55℃ for 30 s, 72℃ for 45 s, final extension 72℃ for 5 min | (36) |
| ermC | TAATCGTGGAATACGGGTTTG AATCGTCAATTCCTGCATGT | 299 | ||
| tetK | GTAGCGACAATAGGTAATAGT GTAGTGACAATAAACCTCCTA | 360 | ||
| tetM | CATATGTCCTGGCGTGTCTA AGTGGAGCGATTACAGAA | 158 | 94℃, 5 min, 30 cycles of 94℃ for 1 min, 57℃ for 1 min, 72℃ for 1 min, | (18) |
| tetL | ATAAATTGTTTCGGGTCGGTAAT AACCAGCCAACTAATGACAATGAT | 1077 | final extension 72℃ for 5 min | |
| tetO | AACTTAGGCATTCTGGCTCAC TCCCACTGTTCCATATCGTCA | 514 | ||
| msrA/B | GCAAATGGTGTAGGTAAGACAACT ATCATGTGATGTAAACAAAAT | 399 | 95℃, 3 min, 35 cycles of 93℃ for 30 s, 55℃ for 2 min, 74℃ for 1 min, final extension 72℃ 90 s | (36) |
| Aac(6′)/aph(2ʺ) | CAGAGCCTTGGGAAGATGAAG CCTCGTGTAATTCATGTTCTGGC | 348 | 94℃, 10 min, 35 cycles of 94℃ for 45 s, 60℃ for 60 s, 72℃ for 60 s, final extension cycle 72℃ for 5 min | (39) |
| norA | TTTGTTTTCAGTGTCAGAATTTATGTTTG GGCTTGGTGAAATATCAGCTATTAAAC | 140 | 94℃, 5 min, 30 cycles of 94℃ for 30 s, 60℃ for 30 s, 72℃ for 60 s, final extension 72℃ for 5 min | (26) |
| cfr | TGA AGT ATA AAG CAG GTT GGG AGT CA ACC ATA TAA TTG ACC ACA AGC AGC | 746 | 94℃, 2 min, 30 cycles of 94℃ for 30 s, 45℃ for 30 s, and 72℃ for 45 s, final extension 72℃ for 1 min | (13) |
| mphC | GAG ACT ACC AAG AAG ACC TGA CG CAT ACG CCG ATT CTC CTG AT | 530 | 95℃, 5 min, 35 cycles of 94℃ for 1 min, 59℃ for 1 min, 72℃ for 2 min, final extension 72℃ for 1 min | (16) |
| sea | TGCATGTTTTCAGAGTTAATC ACGATCAATTTTTACAGC | 544 | ||
| seb | TCTTTGTCGTAAGATAAACTTC GAATGATATTAATTCGCATC | 416 | 94℃, 5 min, 35 cycles of 94℃ for 2 min, 57℃ for 2 min, 72℃ for 1 min, | (22) |
| sec | GACATAAAAGCTAGGAATTT AAATCGGATTAACATTATCCA | 257 | final extension 72℃ for 7 min | |
| sed | TTACTAGTTTGGTAATATCTCCTT CCACCATAACAATTAATGC | 334 | ||
| see | ATAGATAAAGTTAAAACAAGCAA TAACTTACCGTGGACCC | 170 | ||
| tst | ACCCCTGTTCCCTTATCATC TTTTCAGTATTTGTAACGCC | 326 | ||
| pvl | ATCATTAGGTAAAATGTCTGGACATGATCCA GCATCAASTGTATTGGATAGCAAAAGC | 433 | 95℃, 5 min, 30 cycles of 94℃ for 1 min, 55℃ for 30 s, 72℃ for 1 min, | (38) |
| eta | GCAGGTGTTGATTTAGCATT AGATGTCCCTATTTTTGCTG | 93 | final extension at 72℃ for 5 min | |
| etb | ACAAGCAAAAGAATACAGCG GTTTTTGGCTGCTTCTCTTG | 226 |