Table 3.
Effect of SAA on bacterial mutation assay
| Test Article | Dose (µg/plate) | S9 mix | Number of revertant colonies | ||||
|---|---|---|---|---|---|---|---|
| TA97 | TA98 | TA100 | TA1535 | TA102 | |||
| Vehicle control | - | 145.0 ± 2.0 | 38.7 ± 4.5 | 151.7 ± 9.9 | 19.0 ± 7.5 | 252.0 ± 23.5 | |
| + | 128.0 ± 6.0 | 40.0 ± 20.7 | 141.0 ± 25.1 | 16.0 ± 6.9 | 287.0 ± 22.1 | ||
| Positive control | - | 1508.0 ± 10.6① | 247.7 ± 25.5② | 3821.3 ± 398.5③ | 2288.0 ± 246.6③ | 986.7 ± 282.3④ | |
| + | 1228.0 ± 25.0⑤ | 404.0 ± 43.3⑤ | 2612.0 ± 255.9⑤ | 1729.3 ± 93.6⑤ | 1218.3 ± 149.1⑥ | ||
| SAA | 5000 | - | 125.3 ± 20.6 | 28.3 ± 2.1 | 159.0 ± 14.9 | 16.7 ± 4.5 | 246.0 ± 17.6 |
| + | 146.3 ± 3.1 | 42.0 ± 13.9 | 155.0 ± 11.1 | 14.3 ± 2.9 | 304.7 ± 17.4 | ||
| 1000 | - | 143.0 ± 6.0 | 31.7 ± 4.9 | 162.3 ± 23.2 | 15.3 ± 4.9 | 251.3 ± 20.6 | |
| + | 152.0 ± 18.0 | 38.7 ± 6.7 | 161.0 ± 4.4 | 18.7 ± 0.6 | 298.0 ± 24.0 | ||
| 100 | - | 136.7 ± 8.5 | 35.0 ± 7.8 | 160.7 ± 11.0 | 11.7 ± 2.5 | 250.7 ± 13.2 | |
| + | 125.0 ± 33.1 | 45.0 ± 16.8 | 148.0 ± 2.6 | 14.7 ± 4.2 | 295.0 ± 21.7 | ||
| 10 | - | 135.3 ± 10.4 | 34.7 ± 4.0 | 168.3 ± 6.4 | 12.3 ± 4.0 | 238.3 ± 6.7 | |
| + | 140.0 ± 9.0 | 43.0 ± 15.6 | 142.7 ± 3.8 | 16.3 ± 3.8 | 298.7 ± 27.4 | ||
| 1 | - | 137.7 ± 8.5 | 40.0 ± 2.6 | 153.3 ± 2.1 | 17.7 ± 1.5 | 254.0 ± 5.2 | |
| + | 139.0 ± 10.6 | 46.3 ± 11.7 | 129.0 ± 21.0 | 16.7 ± 4.5 | 287.7 ± 13.6 | ||
|
①9-aminoacridine 50 µg/plate ② 2,7-diaminofluorene 20 µg/plate ③Sodium azide 1.5 µg/plate ④Mitomycin C 0.5 µg/plate ⑤2-aminofluorene 50 µg/plate ⑥1,8-dihydroxyanthraquinone 50 µg/plate | |||||||