Fig. 4.

Experimental set-ups for identifying a vocalizing subject. A Identifying a vocalizing subject by neutralizing vocalizations of a stimulus by any of various means, including devocalization, anesthesia, or presenting the subject with bedding from the stimulus’s cage, B placing the stimulus in a plexiglass-covered compartment for recording USVs from the subject’s compartment alone, C using two microphones positioned at opposite ends above the compartments of the interacting animals in a divided arena, D using two microphones positioned at opposite ends of an arena in which nose-to-nose-interacting subjects are placed on separate platforms, E using a four-microphone array for recording vocalizations from multiple animals during free interaction, and F using four microphones while recording from two interacting animals across a gap