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. 2022 Oct 18;11(10):2046. doi: 10.3390/antiox11102046

Table 4.

Individual phenolic compounds identified by UPLC-PDA-MS/MS in J. regia pollen extract.

No. Compound tR λmax [M-H]− m/z Amount
min nm MS MS/MS mg/100 g dw
1 Quercetin 3-O-rutinoside-7-O-glucoside 2.83 255, 350 771 609, 301 0.17 ± 0.00 a
2 Chlorogenic acid * 3.03 299 sh, 327 353 191, 179 0.30 ± 0.01 a
3 Juglanoside B 3.43 257, 317 339 175 1.58 ± 0.03 abc
4 6′-O-acetyl Juglanoside D 3.60 258, 348 401 355, 175 0.66 ± 0.01 ab
5 6′-O-acetyl Juglanoside A 3.70 258, 338 369 323 0.82 ± 0.03 ab
6 Juglanoside D 3.76 262, 355 355 175 0.65 ± 0.03 ab
7 Quercetin 3,7-O-diglucoside 3.84 254, 345 625 463, 301 7.36 ± 0.09 f
8 Quercetin 3-O-sophoroside * 3.89 255, 352 625 301 278.8 ± 1.26 j
9 Quercetin 3-O-glucoside-xyloside 4.25 255, 352 595 301 4.95 ± 0.22 e
10 N-caffeoyl-N-coumaroyl spermidine 4.30 299 sh, 312 452 289, 135 6.80 ± 0.27 f
11 Kaempferol 3-O-sophoroside * 4.36 264, 347 609 285 26.43 ± 1.00 i
12 Kaempferol 3,7-O-diglucoside 4.49 262, 338 609 447, 285 0.94 ± 0.01 ab
13 N,N-dicaffeoyl spermidine 4.58 299 sh, 312 451 271 1.91 ± 0.03 bc
14 Quercetin 3-O-glucoside * 4.64 255, 353 463 301 19.78 ± 0.31 h
15 4′,5,7-Trihydroxy-3,6-dimethoxyflavone-7-O-beta-D-glucoside 4.79 262, 331 491 329 27.37 ± 1.73 i
16 Quercetin 3-O-pentoside 5.05 255, 353 433 301 1.13 ± 0.03 ab
17 N,N-dicoumarylo spermidine 5.10 297 463 273 7.49 ± 0.70 f
18 Quercetin 3-O-pentoside 5.16 255, 353 433 301 4.09 ± 0.32 d
19 Kaempferol 3-O-glucoside * 5.18 262, 350 447 285 2.85 ± 0.22 cd
20 Quercetin 3-O-pentoside 5.22 255, 355 433 301 1.22 ± 0.02 ab
21 Quercetin 3-O-rhamnoside * 5.41 255, 348 447 301 11.91 ± 0.39 g
22 Kaempferol 3-O-rhamnoside 6.12 262, 350 431 285 0.28 ± 0.00 a
23 Unidentified caffeic derivative 6.34 299 sh, 321 501 179 0.61 ± 0.02 ab
24 Kaempferol 3-O-acetyl-glucoside 6.43 262, 338 489 447, 285 0.18 ± 0.01 a
Total 408.3 ± 7.30

Abbreviations: tR, retention time; UV-Vis, ultraviolet-visible; [M-H]−, negative ion values; m/z, mass-to-charge ratio; dw, dry weight; *, compounds identified by standards. Values are expressed as mean ± SD. The statistical significance was analyzed with Duncan’s test. Values marked with different letters indicate statistically significant differences (p < 0.05).